Open AccessModification of methods used in bacteriophage typing of Mycobacterium tuberculosis isolates
Author(s) -
Wilbur Devereux Jones,
Joseph Greenberg
Publication year - 1978
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.7.5.467-469.1978
Subject(s) - phage typing , typing , mycobacterium tuberculosis , bacteriophage , microbiology and biotechnology , strain (injury) , biology , virology , agar , mycobacterium , tuberculosis , bacteria , escherichia coli , medicine , genetics , pathology , anatomy , gene
A procedure in which soft agar overlays were used in bacteriophage-typing Mycobacterium tuberculosis is presented. This safer method uses commercially available media, whereas media presently used must be prepared in the laboratory. Single plaque isolations of the phage BG-1 specifying phage type A and B of M. tuberculosis were readily made by using the modified procedures. This purification and the use of prototype strain Myc 1415 as the indicator host strain have significantly enhanced the ability to discriminate among strains of phage types A and B.