Open AccessLa Crosse virus soluble cell culture antigen
Author(s) -
Helen S. Lindsey,
Richard A. Klimas,
John F. Obijeski
Publication year - 1977
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.6.6.618-626.1977
Subject(s) - antigen , complement fixation test , immunodiffusion , heterologous , biology , virus , virology , microbiology and biotechnology , polyacrylamide gel electrophoresis , precipitin , ouchterlony double immunodiffusion , antibody , biochemistry , immunology , antiserum , serology , gene , enzyme
A virus-free soluble antigen, obtained by ammonium sulfate precipitation of the supernatant fluids of La Crosse virus-infected BHK-21 cell cultures, was more reactive and more specific than infected suckling mouse brain antigen when compared by immunodiffusion and counterelectrophoresis tests. By complement fixation tests, the antigen was cross-reactive with heterologous California group arbovirus hyperimmune mouse ascitic fluids, but to a lesser degree than was the standard sucrose-acetone-extracted infected suckling mouse brain antigen. The major virion nucleocapsid protein of La Crosse virus was found by polyacrylamide gel electrophoresis to be the soluble antigen protein responsible for precipitation in immunodiffusion and counterelectrophoresis tests.