Highly Sensitive Method for Amplification of Human Immunodeficiency Virus Type 2 DNA | Zendy

Florence Damond | Zendy; Ibtissam Loussert-Ajaka | Zendy; Cristian Apetrei | Zendy; Diane Descamps | Zendy; Sandrine Souquière | Zendy; A. Leprêtre | Zendy; Sophie Matheron | Zendy; Françoise BrunVézinet | Zendy; François Simon | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Highly Sensitive Method for Amplification of Human Immunodeficiency Virus Type 2 DNA

Author(s) -

Florence Damond,

Ibtissam Loussert-Ajaka,

Cristian Apetrei,

Diane Descamps,

Sandrine Souquière,

A. Leprêtre,

Sophie Matheron,

Françoise BrunVézinet,

François Simon

Publication year - 1998

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.36.3.809-811.1998

Subject(s) - primer (cosmetics) , biology , virology , nested polymerase chain reaction , polymerase chain reaction , microbiology and biotechnology , long terminal repeat , dna , peripheral blood mononuclear cell , virus , primer dimer , genome , provirus , gene , genetics , chemistry , multiplex polymerase chain reaction , organic chemistry , in vitro

We evaluated a new human immunodeficiency virus type 2 (HIV-2) DNA amplification strategy based on peripheral blood mononuclear cell long PCR (XL PCR) followed by nested PCR amplification. The primers used were located in the highly conserved long terminal repeat and in thepol regions of the genome. Five primer pairs corresponding to different regions of the HIV-2env gene were used in the nested step. Samples from 42 patients were tested, which yielded positive amplification with at least two primer pairs in 40 (95%) samples. A primer pair (EB2/EB5) located on the V3 region succeeded in amplifying proviral DNA in 40 samples.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research