Rapid susceptibility testing of fungi by flow cytometry using vital staining | Zendy

Christoph Wenisch | Zendy; Ken F. Linnau | Zendy; Bernhard Parschalk | Zendy; Konstantin ZedtwitzLiebenstein | Zendy; Aristidis Georgopoulos | Zendy

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Rapid susceptibility testing of fungi by flow cytometry using vital staining

Author(s) -

Christoph Wenisch,

Ken F. Linnau,

Bernhard Parschalk,

Konstantin ZedtwitzLiebenstein,

Aristidis Georgopoulos

Publication year - 1997

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.35.1.5-10.1997

Subject(s) - flow cytometry , fluconazole , ketoconazole , flucytosine , amphotericin b , candida albicans , microbiology and biotechnology , yeast , biology , staining , in vivo , antifungal , vital stain , biochemistry , genetics

A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results comparable to those obtained by the standard M27 procedure for amphotericin B, flucytosine, fluconazole, and ketoconazole. Whether the flow cytometry antifungal susceptibility test results correlate with the in vivo activities of the drugs remains to determined.

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