Rapid antigen detection assay for identification of Burkholderia (Pseudomonas) pseudomallei infection | Zendy

A Anuntagool | Zendy; Pakamas Intachote | Zendy; Pimjai Naigowit | Zendy; Stitaya Sirisinha | Zendy

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Rapid antigen detection assay for identification of Burkholderia (Pseudomonas) pseudomallei infection

Author(s) -

A Anuntagool,

Pakamas Intachote,

Pimjai Naigowit,

Stitaya Sirisinha

Publication year - 1996

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.34.4.975-976.1996

Subject(s) - melioidosis , burkholderia pseudomallei , microbiology and biotechnology , antigen , burkholderia , monoclonal antibody , pseudomonas , biology , antibody , virology , bacteria , immunology , genetics

A simple antigen detection test was developed for rapid diagnosis of melioidosis caused by Burkholderia (Pseudomonas) pseudomallei infection. The method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for B. pseudomallei. The results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.

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