Open AccessColorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples
Author(s) -
S N Cho,
G M van der Vliet,
SoonJung Park,
Seung Hyuk Baik,
S K Kim,
Yunsop Chong,
A H Kolk,
Paul R. Klatser,
Jung Dae Kim
Publication year - 1995
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.33.3.752-754.1995
Subject(s) - mycobacterium tuberculosis , biotinylation , sputum , biology , microbiology and biotechnology , dna , hybridization probe , polymerase chain reaction , nucleic acid thermodynamics , dna–dna hybridization , mycobacterium , virology , tuberculosis , base sequence , gene , bacteria , genetics , pathology , medicine
We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
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