Rapid screening for resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples | Zendy

Giuseppe Gerna | Zendy; Antonella Sarasini | Zendy; Elena Percivalle | Zendy; Maurizio Zavattoni | Zendy; Fausto Baldanti | Zendy; Maria Grazia Revello | Zendy

Open Access

Rapid screening for resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples

Author(s) -

Giuseppe Gerna,

Antonella Sarasini,

Elena Percivalle,

Maurizio Zavattoni,

Fausto Baldanti,

Maria Grazia Revello

Publication year - 1995

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.33.3.738-741.1995

Subject(s) - foscarnet , ganciclovir , human cytomegalovirus , virology , cytomegalovirus , biology , betaherpesvirinae , drug resistance , herpesviridae , virus , blood culture , microbiology and biotechnology , immunology , viral disease , antibiotics

A rapid screening assay for the detection of resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples was developed by using single doses of both drugs and an immediate-early antigen plaque reduction assay. Results of the rapid assay with peripheral blood leukocytes as the viral inoculum were compared with those of a conventional assay with cell-free virus from the relevant viral isolates. Both assays gave overlapping results. The rapid assay offers the following major advantages: (i) it provides results within 4 to 6 days, (ii) it can be performed with peripheral blood leukocytes, and (iii) it reliably detects drug-resistant human cytomegalovirus strains.

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