Specific amplification of Rickettsia japonica DNA from clinical specimens by PCR | Zendy

Yumiko Furuya | Zendy; Takashi Katayama | Zendy; Yuki Yoshida | Zendy; Ikuo Kaiho | Zendy

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Specific amplification of Rickettsia japonica DNA from clinical specimens by PCR

Author(s) -

Yumiko Furuya,

Takashi Katayama,

Yuki Yoshida,

Ikuo Kaiho

Publication year - 1995

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.33.2.487-489.1995

Subject(s) - rickettsiosis , biology , polymerase chain reaction , primer (cosmetics) , virology , rickettsia , genomic dna , spotted fever , rickettsiales , dna , gene , genetics , virus , chemistry , organic chemistry

The gene encoding the 17,000-molecular-weight genus-common antigen (17K genus-common antigen) has been cloned and sequenced from Rickettsia japonica. The primer pair used for PCR was designed from this sequence. A 357-bp fragment was observed by amplifying the genomic DNA from R. japonica and also the DNA from blood clots of patients with spotted fever group rickettsiosis. The results indicated that this method is suitable for the diagnosis of spotted fever group rickettsiosis in Japan.

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