Open AccessRapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis
Author(s) -
Richard V. Goering,
Mark A. Winters
Publication year - 1992
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.30.3.577-580.1992
Subject(s) - restriction enzyme , gram positive cocci , agarose gel electrophoresis , staphylococcus epidermidis , gel electrophoresis , agarose , biology , gram , pulsed field gel electrophoresis , microbiology and biotechnology , restriction map , isolation (microbiology) , micrococcaceae , dna , bacteria , genetics , staphylococcus aureus , genotype , base sequence , gene
We report a rapid method for the isolation of intact chromosomal DNA from gram-positive cocci that is suitable for in situ restriction endonuclease digestion in agarose blocks. When combined with a rapid field inversion gel electrophoresis protocol, this approach allows the preparation and electrophoretic analysis of chromosomal restriction fragments produced by rare-cutting enzymes in a total time period of 2 days from start to finish. The utility of the method is demonstrated in the epidemiological evaluation of Staphylococcus epidermidis clusters from two hospitals as well as of additional representative staphylococci and enterococci.
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