Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients | Zendy

Donato Zipeto | Zendy; Maria Grazia Revello | Zendy; Enrico Maria Silini | Zendy; M. Parea | Zendy; Elena Percivalle | Zendy; Maurizio Zavattoni | Zendy; G. Milanesi | Zendy; Giuseppe Gerna | Zendy

Open Access

Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients

Author(s) -

Donato Zipeto,

Maria Grazia Revello,

Enrico Maria Silini,

M. Parea,

Elena Percivalle,

Maurizio Zavattoni,

G. Milanesi,

Giuseppe Gerna

Publication year - 1992

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.30.2.527-530.1992

Subject(s) - human cytomegalovirus , viremia , polymerase chain reaction , concordance , virology , betaherpesvirinae , cytomegalovirus , real time polymerase chain reaction , herpesviridae , biology , immunology , clinical significance , viral disease , medicine , virus , pathology , gene , bioinformatics , biochemistry

The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised patients. Of the 86 patients, 23 underwent clinical and virologic follow-up for HCMV infection. In parallel, blood samples were examined for viremia and antigenemia. Concordant results between PCR and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 samples (most from patients with HCMV infection) were positive by PCR alone. PCR is a new powerful tool for detection of HCMV infections in blood samples from immunocompromised patients. However, its clinical significance appears to be restricted to the indication of a risk of reactivation of HCMV infection.

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