Open AccessImproved method for production of attenuated Venezuelan equine encephalomyelitis (TC-83 strain) vaccine
Author(s) -
Francis E. Cole,
Carl E. Pedersen,
David M. Robinson,
Gerald A. Eddy
Publication year - 1976
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.3.4.460-462.1976
Subject(s) - virology , virulence , attenuated vaccine , strain (injury) , embryo , biology , potency , virus , cell culture , serial passage , encephalomyelitis , microbiology and biotechnology , immunology , in vitro , genetics , gene , anatomy , multiple sclerosis
Primary chicken embryo cell cultures were evaluated a s an alternate cell system for the production of attenuated Venezuelan equine encephalomyelitis (TC-83 strain) vaccine. The TC-83 strain virus was shown to remain stable during 10 serial passages in chicken embryo cell culture with regard to plaque size and morphology, virus yield, potency, and virulence for mice and hamsters.