Open AccessComparison of Chlamydia subgroup A detection from clinical specimens after 40 and 64 hours of incubation in 5-iodo-2-deoxyuridine-treated McCoy's cells
Author(s) -
J E Jonhson,
Thomas F. Smith
Publication year - 1976
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.3.3.334-338.1976
Subject(s) - chlamydia trachomatis , chlamydia , deoxyuridine , incubation , incubation period , chlamydiaceae , staining , chlamydiales , genitourinary system , biology , pathology , medicine , microbiology and biotechnology , virology , immunology , dna , biochemistry , genetics
The time course of formation of inclusion bodies produced by Chlamydia in 5-iodo-2-deoxyuridine (IUdR)-treated McCoy's cells was studied with the use of a known isolate of Chlamydia trachomatis D/UW-184/Ur and 47 frozen clinical urethral specimens previously shown to be either positive or negative for chlamydial inclusions after 3 days of incubation. Subsequent examination of 369 clinical specimens from the genitourinary tract over a 6-month period revealed 47 (13%) Chlamydia-positive cultures, all of which demonstrated inclusion bodies by iodine staining at 40 and 64 h postinoculation. Another 146 similar detected by iodine staining from 22 (15%). This study indicates that, although Chlamydia subgroup A inclusions are larger at 64 h, they can be readily detected from clinical specimens in IUdR-treated McCoy's cells at 40 h postinfection.