Open AccessTitration of Newcastle disease virus and its neutralizing antibodies in microplates by a modified hemadsorption and hemadsorption inhibition method
Author(s) -
Ness-Ziona,
Israel Israel
Publication year - 1976
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.3.3.227-232.1976
Subject(s) - infectivity , virology , newcastle disease , antibody , titration , virus , neutralizing antibody , hemagglutination , hemagglutination assay , neutralization , microtiter plate , biology , chemistry , microbiology and biotechnology , titer , immunology , inorganic chemistry
Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and compared to the plaque neutralization and microhemagglutination inhibition test. Reproducibility of the microneutralization test was statistically analyzed.