Open AccessDetection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method
Author(s) -
D. Marmet,
N. Bornstein,
J Fleurette
Publication year - 1990
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.28.12.2828-2829.1990
Subject(s) - legionella , chromatography , high performance liquid chromatography , hydrolysis , chemistry , benzoic acid , microbiology and biotechnology , bacteria , biochemistry , biology , genetics
A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
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