Purification and characterization of an Aeromonas hydrophila hemolysin | Zendy

Tsutomu Asao | Zendy; S Kozaki | Zendy; Keiko Kato | Zendy; Y Kinoshita | Zendy; Kinya Otsu | Zendy; Takehiro Uemura | Zendy; Genji Sakaguchi | Zendy

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Purification and characterization of an Aeromonas hydrophila hemolysin

Author(s) -

Tsutomu Asao,

S Kozaki,

Keiko Kato,

Y Kinoshita,

Kinya Otsu,

Takehiro Uemura,

Genji Sakaguchi

Publication year - 1986

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.24.2.228-232.1986

Subject(s) - hemolysin , isoelectric focusing , microbiology and biotechnology , aeromonas hydrophila , immunodiffusion , biology , antigen , aeromonas , chemistry , bacteria , biochemistry , virulence , immunology , enzyme , genetics , gene

A hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was purified by sulfopropyl-Sephadex C-25 chromatography at pH 5.0. This hemolysin caused fluid accumulation in infant mouse intestines and rabbit intestinal loops and killed Vero cells, as did the hemolysin produced by strain AH-1, isolated from a diarrheal case. In polyacrylamide gel electrophoreses at pHs 4.0 and 9.4 and in thin-layer isoelectric focusing, CA-11 hemolysin migrated as a single band to a position different from that of AH-1 hemolysin. Immunodiffusion tests indicated that CA-11 hemolysin was immunologically related to AH-1 hemolysin but possessed unique antigenic determinants. Neutralization tests with antihemolysin sera also demonstrated immunological cross-reactivity between AH-1 and CA-11 hemolysins. These results apparently indicate that the hemolysins produced by the two strains of A. hydrophila are immunologically and physicochemically different from each other.

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