Detection of pneumococci in the upper respiratory tract: comparison of media and culture techniques | Zendy

Roy M. RobinsBrowne | Zendy; A Kharsany | Zendy; U Ramsaroop | Zendy

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Detection of pneumococci in the upper respiratory tract: comparison of media and culture techniques

Author(s) -

Roy M. RobinsBrowne,

A Kharsany,

U Ramsaroop

Publication year - 1982

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.16.1.1-3.1982

Subject(s) - microbiology and biotechnology , streptococcus pneumoniae , agar plate , agar , horse , biology , throat culture , gentamicin , throat , bacteria , streptococcus , antibiotics , paleontology , genetics , anatomy

A survey for carriers of Streptococcus pneumoniae among 573 children was undertaken. Throat and nasopharyngeal cultures from 303 children disclosed pneumococci in 89 (29.4%) nasopharyngeal swabs but in only 8 (2.6%) throat specimens. Carriers of pneumococci were detected with frequencies of 84.4 and 79.9% on aerobically incubated 5% horse blood agar with a 5-micrograms optochin disk and on 5% horse blood agar with 5 mg of gentamicin per liter, respectively. Anaerobically incubated 5% horse blood agar yield pneumococci from 78.2% of 179 carriers. We consider the examination of nasopharyngeal secretions for optochin-susceptible alpha-hemolytic bacteria to be the most sensitive and convenient means for detecting carriers of pneumococci.

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