Enhancement of coagglutination reactions of the Phadebact gonococcus test by ethylenediaminetetraacetate and ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetate | Zendy

I Izakson | Zendy; Stephen Morse | Zendy

Open Access

Enhancement of coagglutination reactions of the Phadebact gonococcus test by ethylenediaminetetraacetate and ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetate

Author(s) -

I Izakson,

Stephen Morse

Publication year - 1981

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.14.3.261-265.1981

Subject(s) - ethylenediaminetetraacetic acid , ethylene glycol , chemistry , nuclear chemistry , ether , chelation , citric acid , divalent , medicinal chemistry , inorganic chemistry , organic chemistry

Incubation of gonococci under conditions optimal for autolysis resulted in increased sensitivity and enhancement of the coagglutination reaction of the Phadebact gonococcus test. These conditions included an alkaline pH (pH 8.3) and the presence of divalent cation chelators such as ethylenediaminetetraacetic acid or ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid. Heating cell suspensions at 90 degrees C for 15 min before assay by coagglutination produced a further increase in sensitivity and enhancement of the reaction. Gonococcal lipopolysaccharide was found to be an important antigen in these coagglutination reactions. The detection of lipopolysaccharide was markedly enhanced by the addition of chelating agents.

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