Rapid method for identification of group B streptococci in neonatal blood cultures
Author(s) -
R L Holmes,
W A Harada
Publication year - 1981
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.13.2.279-282.1981
Subject(s) - streptococcus agalactiae , streptococcus , microbiology and biotechnology , blood culture , latex fixation test , group b , streptococcaceae , biology , medicine , immunology , bacteria , antibody , antibiotics , genetics
A rapid technique used for the identification of Streptococcus agalactiae, Lancefield group B, from the blood cultures of two neonatal infants is reported. The method utilized the Phadebact Streptococcus Test System (Pharmacia Diagnostics, Piscataway, N.J.) and the supernatant from 13- and 14-h blood cultures. Additional studies with simulated neonatal blood cultures revealed that this method was reproducible. Additional studies also revealed that some non-specific agglutination did occur, which could not be eliminated with dithiothreitol, but was visibly reduced by treatment with soluble staphylococcal protein A.
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