Open AccessA Whole-Genome-Based Gene-by-Gene Typing System for Standardized High-Resolution Strain Typing of Bacillus anthracis
Author(s) -
Mostafa Y. Abdel-Glil,
Alexandra Chiaverini,
Giuliano Garofolo,
Antonio Fasanella,
Antonio Parisi,
Dag Harmsen,
Keith A. Jolley,
Mandy C. Elschner,
Herbert Tomaso,
Jörg Linde,
Domenico Galante
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02889-20
Subject(s) - subtyping , bacillus anthracis , typing , multilocus sequence typing , biology , genome , genotyping , phylogenetic tree , whole genome sequencing , snp array , genetics , single nucleotide polymorphism , outbreak , computational biology , genotype , gene , virology , computer science , bacteria , programming language
Whole-genome sequencing (WGS) has been established for bacterial subtyping and is regularly used to study pathogen transmission, to investigate outbreaks, and to perform routine surveillance. Core-genome multilocus sequence typing (cgMLST) is a bacterial subtyping method that uses WGS data to provide a high-resolution strain characterization. This study aimed at developing a novel cgMLST scheme forBacillus anthracis , a notorious pathogen that causes anthrax in livestock and humans worldwide.