Open AccessDetection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
Author(s) -
Melanie A. MacMullan,
Prithivi Chellamuthu,
Aubree Mades,
Sudipta Sekhar Das,
Fred Turner,
Vladimir I. Slepnev,
Albina Ibrayeva
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02510-20
Subject(s) - covid-19 , antibody , virology , medicine , coronavirus , immunology , pathology , infectious disease (medical specialty) , disease , outbreak
Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.