Open AccessVisualizing Borrelia burgdorferi Infection Using a Small-Molecule Imaging Probe
Author(s) -
Madeline G Sell,
David A. Alcorta,
Andrew E. Padilla,
Dakota W. Nollner,
Nicole R. Hasenkampf,
Havard S. Lambert,
Monica E. Embers,
Neil L. Spector
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02313-20
Subject(s) - borrelia burgdorferi , biology , microbiology and biotechnology , bacteria , pathogen , borrelia , in vivo , small molecule , lyme disease , virology , biochemistry , immunology , antibody , genetics
In vivo diagnostic imaging of bacterial infections is currently reliant on targeting their metabolic pathways, an ineffective method to identify microbial species with low metabolic activity. Here, we establish HS-198 as a small-molecule fluorescent conjugate that selectively targets the highly conserved bacterial protein HtpG (high-temperature protein G), withinBorrelia burgdorferi , the bacterium responsible for Lyme disease.