Open AccessHigh Prevalence of Coinfecting Enteropathogens in Suspected Rotavirus Vaccine Breakthrough Cases
Author(s) -
Ceren Simsek,
Mandy Bloemen,
Daan Jansen,
Leen Beller,
Patrick Descheemaeker,
Marijke Reynders,
Marc Van Ranst,
Jelle Matthijnssens
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01236-21
Subject(s) - rotavirus , virology , rotavirus vaccine , reoviridae , rotavirus infections , metagenomics , medicine , biology , virus , gene , biochemistry
Despite the global use of rotavirus vaccines, vaccine breakthrough cases remain a pediatric health problem. In this study, we investigated suspected rotavirus vaccine breakthrough cases using next-generation sequencing (NGS)-based viral metagenomics (n = 102) and a panel of semiquantitative reverse transcription-PCR (RT-qPCR) (n = 92) targeting known enteric pathogens.