Open AccessNanopore Sequencing for Mycobacterium tuberculosis: a Critical Review of the Literature, New Developments, and Future Opportunities
Author(s) -
Anzaan Dippenaar,
Sander N Goossens,
Melanie Grobbelaar,
Selien Oostvogels,
Bart Cuypers,
Kris Laukens,
Conor J. Meehan,
Robin M. Warren,
Annelies Van Rie
Publication year - 2022
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00646-21
Subject(s) - mycobacterium tuberculosis , tuberculosis , dna sequencing , nanopore sequencing , whole genome sequencing , software , data science , computational biology , computer science , biology , medicine , genome , genetics , gene , dna , pathology , programming language
The next-generation, short-read sequencing technologies that generate comprehensive, whole-genome data with single nucleotide resolution have already advanced tuberculosis diagnosis, treatment, surveillance, and source investigation. Their high costs, tedious and lengthy processes, and large equipment remain major hurdles for research use in high tuberculosis burden countries and implementation into routine care.