Open AccessAnalytical Performance Characteristics of a New Transcription-Mediated Amplification Assay for Treponema pallidum
Author(s) -
Damon Getman,
Mike Lin,
Nesreen Barakat,
Rhonda Skvoretz,
Charmie Godornes,
Paul D. Swenson,
Ashley Nenninger,
Matthew R. Golden,
Sheila A. Lukehart
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00511-21
Subject(s) - treponema , biology , microbiology and biotechnology , transcription (linguistics) , virology , syphilis , human immunodeficiency virus (hiv) , linguistics , philosophy
This study evaluated the performance characteristics of a new research-use-only transcription-mediated amplification (TMA) assay for the detection of rRNA fromTreponema pallidum . Analytical sensitivity determined using dark-field microscopy-quantitatedT. pallidum was 1.4 organisms/ml (95% confidence interval [CI], 0.7 to 6.33 organisms/ml).