Open AccessDiagnosing Pulmonary Tuberculosis by Using Sequence-Specific Purification of Urine Cell-Free DNA
Author(s) -
Amy Oreskovic,
Nuttada Panpradist,
Diana Marangu,
Mduduzi W. Ngwane,
Zanele P. Magcaba,
Sindiswa Ngcobo,
Zinhle Ngcobo,
David Horné,
Douglas P. Wilson,
Adrienne E. Shapiro,
Paul K. Drain,
Barry R. Lutz
Publication year - 2021
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00074-21
Subject(s) - urine , tuberculosis , biomarker , dna , cell free fetal dna , urine sample , pulmonary tuberculosis , medicine , biology , virology , pathology , genetics , pregnancy , fetus , prenatal diagnosis
Transrenal urine cell-free DNA (cfDNA) is a promising tuberculosis (TB) biomarker, but is challenging to detect because of the short length (<100 bp) and low concentration of TB-specific fragments. We aimed to improve the diagnostic sensitivity of TB urine cfDNA by increasing recovery of short fragments during sample preparation.