Chemical and Serological Relationships Between the Heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii | Zendy

Susan E. Birnbaum | Zendy; Lewis F. Affronti | Zendy

Open Access

Chemical and Serological Relationships Between the Heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii

Author(s) -

Susan E. Birnbaum,

Lewis F. Affronti

Publication year - 1968

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.95.2.559-564.1968

Subject(s) - mycobacterium kansasii , mycobacterium tuberculosis , polysaccharide , cell wall , microbiology and biotechnology , biology , mycobacterium , ultracentrifuge , antigen , tuberculosis , bacteria , biochemistry , immunology , medicine , genetics , pathology

The identity of a heteropolysaccharide from cell walls ofMycobacterium tuberculosis H37Ra with Seibert's tuberculopolysaccharide I was demonstrated by thin-layer chromatography, chemical analysis, and antigenic tests. The polysaccharide ofM. kansasii was shown to be identical with that ofM. tuberculosis . Defatted cells were disintegrated by ultrasonic treatment in the presence of glass beads; cell walls were obtained by differential ultracentrifugation. Ethyl alcohol-precipitated carbohydrate extracts were analyzed for protein and nucleic acid; these impurities were removed. Tuberculopolysaccharide I from the mycobacterial culture filtrate is probably derived from a lipopolysaccharide of the cell wall, which is partially removed by chloroform in the intact state. Alkaline extraction releases additional polysaccharide, in varying degrees of association with cell wall murein.

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