Antibiotic Action of Griseofulvin on Dermatophytes

El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.),W. L. McLellan, Jr., and J. O. Lampen . Antibiotic action of griseofulvin on dermatophytes. J. Bacteriol.89: 557–563. 1965.—The concentrations of griseofulvin required to inhibit growth and to produce the characteristic morphological distortions were determined for dermatophytes (highly sensitive), fungal plant pathogens (moderately sensitive), filamentous nonpathogenic fungi (poorly sensitive), and for yeasts andEscherichia coli (insensitive). Addition of griseofulvin to small inocula of the dermatophytesMicrosporum gypseum andTrichophyton mentagrophytes produced complete and apparently permanent growth inhibition. If the antibiotic was added to actively growing cultures, the inhibition was only temporary, even with the most sensitive dermatophytes. During growth inhibition, griseofulvin temporarily halted the net synthesis of protein and nucleic acids, and of the amino acid and nucleotide pools. It decreased substantially the incorporation of C14 -uridine or C14 -thymidine into nucleic acids ofM. gypseum , but not that of C14 -leucine or C14 -valine into protein. With a less sensitive culture,T. mentagrophytes x8, the uptake of uridine was inhibited only to a slight extent; the incorporation of leucine was unaffected. A partial protective effect of pruine nucleotides against growth inhibition by griseofulvin was observed with one strain ofT. mentagrophytes , but not with another strain or withM. gypseum .