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Beardsley, Robert E. (Manhattan College, New York, N. Y.). Amino acid cross resistance inAgrobacterium tumefaciens . J. Bacteriol.84: 1237–1240. 1962.—Resistant clones selected on medium supplemented with glycine were also resistant tod -methionine,d -valine,dl -norleucine, anddl -serine. Cross resistance was similarly exhibited by clones selected ond -methionine,d -valine, ordl -norleucine. Two types of resistant organisms were observed. One produced colonies containing normal rods on selection medium. The other produced translucent colonies containing L forms. Both grew as typical rods in unsupplemented medium. Some resistant clones did not produce a temperate phage carried by the parental strain, but these retained immunity to homologous phage. The toxicity ofd -methionine andd -valine for nonresistant bacteria is not reversed by thel isomers. The lethal effects of toxic amino acids are additive.

AMINO ACID CROSS RESISTANCE IN AGROBACTERIUM TUMEFACIENS