Open AccessPhysical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
Author(s) -
Ryuichi Hirota,
Akira Yamagata,
Junichi Kato,
Akio Kuroda,
Tsukasa Ikeda,
Noboru Takiguchi,
Hisao Ohtake
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.3.825-828.2000
Subject(s) - nitrosomonas , biology , microbiology and biotechnology , ammonia monooxygenase , gene , hydroxylamine , nucleotide , coding region , nucleic acid sequence , genetics , biochemistry , archaea , nitrite , ecology , nitrate
Pulsed-field gel electrophoresis ofPme I digests of theNitrosomonas sp. strain ENI-11 chromosome produced four bands ranging from 1,200 to 480 kb in size. Southern hybridizations suggested that a 487-kbPme I fragment contained two copies of theamoCAB genes, coding for ammonia monooxygenase (designatedamoCAB1 andamoCAB2 ), and three copies of thehao gene, coding for hydroxylamine oxidoreductase (hao1 ,hao2 , andhao3 ). In this DNA fragment,amoCAB1 andamoCAB2 were about 390 kb apart, whilehao1 ,hao2 , andhao3 were separated by at least about 100 kb from each other. Interestingly,hao1 andhao2 were located relatively close toamoCAB1 andamoCAB2 , respectively. DNA sequence analysis revealed thathao1 andhao2 shared 160 identical nucleotides immediately upstream of each translation initiation codon. However,hao3 showed only 30% nucleotide identity in the 160-bp corresponding region.