Open AccessThe Synechococcus Strain PCC 7942 glnN Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen Chlorosis
Author(s) -
Jörg Sauer,
Ulrike Dirmeier,
Karl Forchhammer
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.19.5615-5619.2000
Subject(s) - biology , glutamine synthetase , mutant , glutamine , synechococcus , chlorosis , gene , strain (injury) , biochemistry , cloning (programming) , cyanobacteria , microbiology and biotechnology , promoter , gene product , transcription (linguistics) , gene expression , genetics , amino acid , bacteria , botany , anatomy , computer science , programming language , linguistics , philosophy
We report the cloning and sequencing of theglnN gene encoding a class III glutamine synthetase from the cyanobacteriumSynechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression ofglnN is impaired in NtcA- and PII -deficient mutants. The only parameter which was negatively affected in theglnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.