Open AccessIdentification of a Second Region of the Spo0A Response Regulator of Bacillus subtilis Required for Transcription Activation
Author(s) -
Dean A. RoweMagnus,
Martin J. Richer,
George B. Spiegelman
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.15.4352-4355.2000
Subject(s) - bacillus subtilis , operon , biology , psychological repression , regulator , response regulator , transcription (linguistics) , transcription factor , promoter , genetics , repressor , amino acid , microbiology and biotechnology , gene , mutant , gene expression , bacteria , linguistics , philosophy
Deletion of the 10 C-terminal amino acids of theBacillus subtilis response regulator Spo0A or valine substitution at D258 and L260 resulted in a sporulation-negative phenotype and loss of in vivo activation of thespoIIG andspoIIA operon promoters. Repression of theabrB promoter was not affected by the mutations. In combination with the previously characterized mutation (A257V), the results identify amino acids at positions 257, 258, and 260 as being required for transcription activation by Spo0A.