Open AccessContribution of Cysteine Desulfurase (NifS Protein) to the Biotin Synthase Reaction of Escherichia coli
Author(s) -
Tatsuya Kiyasu,
Akira Asakura,
Yoshie Nagahashi,
Tatsuo Hoshino
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.10.2879-2885.2000
Subject(s) - biotin , cysteine , escherichia coli , biochemistry , biology , enzyme , gene
The contribution of cysteine desulfurase, the NifS protein ofKlebsiella pneumoniae and the IscS protein ofEscherichia coli , to the biotin synthase reaction was investigated in in vitro and in vivo reaction systems withE. coli . When thenifS andnifU genes ofK. pneumoniae were coexpressed inE. coli , NifS and NifU proteins in complex (NifU/S complex) and NifU monomer forms were observed. Both the NifU/S complex and the NifU monomer stimulated the biotin synthase reaction in the presence ofl -cysteine in an in vitro reaction system. The NifU/S complex enhanced the production of biotin from dethiobiotin by the cells growing in an in vivo reaction system. Moreover, the IscS protein ofE. coli stimulated the biotin synthase reaction in the presence ofl -cysteine in the cell-free system. These results strongly suggest that cysteine desulfurase participates in the biotin synthase reaction, probably by supplying sulfur to the iron-sulfur cluster of biotin synthase.