Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans : Radiation Sensitivities of Catalase ( katA ) and Superoxide Dismutase ( sodA ) Mutants | Zendy

Lye Meng Markillie | Zendy; Susan M. Varnum | Zendy; Preston Hradecky | Zendy; KwongKwok Wong | Zendy

Open Access

Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans : Radiation Sensitivities of Catalase ( katA ) and Superoxide Dismutase ( sodA ) Mutants

Author(s) -

Lye Meng Markillie,

Susan M. Varnum,

Preston Hradecky,

KwongKwok Wong

Publication year - 1999

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.181.2.666-669.1999

Subject(s) - deinococcus radiodurans , superoxide dismutase , mutagenesis , biology , mutant , radioresistance , catalase , deinococcus , microbiology and biotechnology , bacteria , oxidative stress , biochemistry , genetics , gene , cell culture

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA ) and superoxide dismutase (sodA ) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

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