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Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA ) and superoxide dismutase (sodA ) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

journal of bacteriology

Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans : Radiation Sensitivities of Catalase ( katA ) and Superoxide Dismutase ( sodA ) Mutants