Comparison of the Sensitivities of Two Escherichia coli Genes to In Vivo Variation of Lrp Concentration | Zendy

Changfeng Chen | Zendy; E. B. Newman | Zendy

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Comparison of the Sensitivities of Two Escherichia coli Genes to In Vivo Variation of Lrp Concentration

Author(s) -

Changfeng Chen,

E. B. Newman

Publication year - 1998

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.180.3.655-659.1998

Subject(s) - biology , escherichia coli , plasmid , mutant , gene , microbiology and biotechnology , transcription (linguistics) , overproduction , in vivo , genetics , linguistics , philosophy

Transcription of theEscherichia coli genesserA andgltBDF depends on the leucine-responsive regulatory protein, Lrp, and is very much decreased in anlrp mutant. By the use of an Lrp-deficient host and thelrp gene cloned under a plasmid-borne arabinose pBAD promoter, we varied the amount of Lrp present in the cell and showed that both genes were transcribed in proportion to the amount of Lrp synthesized. The affinity ofserA for Lrp was four to five times greater than the affinity ofgltD . Overproduction of Lrp was lethal to the cell.

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