Open AccessCharacterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12
Author(s) -
Yûsuke Saito,
Fumiko Uraki,
Satoshi Nakajima,
Ayumi Asaeda,
Katsushige Ono,
Kihei Kubo,
Kazuo Yamamoto
Publication year - 1997
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.179.11.3783-3785.1997
Subject(s) - endonuclease , biology , escherichia coli , ap endonuclease , mutant , dna (apurinic or apyrimidinic site) lyase , microbiology and biotechnology , dna , ap site , biochemistry , genetics , gene
The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that spontaneous mutagenic lesions are repaired mainly by endonuclease III.