Open AccessCloning of a cryV-type insecticidal protein gene from Bacillus thuringiensis: the cryV-encoded protein is expressed early in stationary phase
Author(s) -
Kristy Kostichka,
Gregory W. Warren,
Martha A. Mullins,
Alex Mullins,
Narendra Palekar,
Joyce A. Craig,
Michael G. Koziel,
Juan José Estruch
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.7.2141-2144.1996
Subject(s) - biology , bacillus thuringiensis , gene , signal peptide , cloning (programming) , peptide sequence , hspa2 , protein sequencing , microbiology and biotechnology , genetics , bacteria , computer science , programming language
A CryV-type protein (CGCryV) has been isolated from supernatant fluids of Bacillus thuringiensis AB88 cultures. Previous reports have suggested the cryptic nature of the cryV-type genes on the basis of the absence of CryV-type proteins in parasporal crystals. The CryV-type protein reported here is expressed early in stationary phase, and evidence indicates that it is an exported protein. Analysis of the deduced protein sequence from this gene reveals the presence of an N-terminal domain that likely acts as a signal peptide. The CGCryV protein is the first reported case of a delta-endotoxin being a secreted protein, which may influence the biological relevance of these proteins.