Open AccessApramycin resistance as a selective marker for gene transfer in mycobacteria
Author(s) -
Eric Paget,
Julian Davies
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.21.6357-6360.1996
Subject(s) - biology , mycobacterium smegmatis , electroporation , plasmid , cloning (programming) , mycobacterium bovis , mycobacterium tuberculosis , gene , shuttle vector , gene transfer , microbiology and biotechnology , genetics , tuberculosis , recombinant dna , vector (molecular biology) , medicine , pathology , computer science , programming language
We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.