Open AccessGenetic control of the resistance to phage C1 of Escherichia coli K-12
Author(s) -
Н. А. Лихачева,
Victor V. Samsonov,
Victor V. Samsonov,
S. P. Sineoky
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.17.5309-5315.1996
Subject(s) - biology , periplasmic space , escherichia coli , open reading frame , gene product , gene , bacterial outer membrane , lytic cycle , bacteriophage , genetics , microbiology and biotechnology , peptide sequence , gene expression , virus
Escherichia coli K-12 lytic phage C1 was earlier isolated in our laboratory. Its adsorption is controlled by at least three bacterial genes: dcrA, dcrB, and btuB. Our results provide evidence that the dcrA gene located at 60 min on the E. coli genetic map is identical to the sdaC gene. This gene product is an inner membrane protein recently identified as a putative specific serine transporter. The dcrB gene, located at 76.5 min, encodes a 20-kDa processed periplasmic protein, as determined by maxicell analysis, and corresponds to a recently determined open reading frame with a previously unknown function. The btuB gene product is known to be an outer membrane receptor protein responsible for adsorption of BF23 phage and vitamin B12 uptake. According to our data the DcrA and DcrB proteins are not involved in these processes. However, the DcrA protein probably participates in some cell division steps.