Open AccessActivation of the dephosphorylation of nitrogen regulator I-phosphate of Escherichia coli
Author(s) -
Ju Liu,
Boris Magasanik
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.4.926-931.1995
Subject(s) - dephosphorylation , biology , phosphate , regulator , phosphorylation , biochemistry , transcription (linguistics) , kinase , microbiology and biotechnology , phosphatase , gene , linguistics , philosophy
The transcription of sigma 54 RNA polymerase-dependent nitrogen-regulated genes is activated by nitrogen regulator I (NRI)-phosphate. The kinase NRII is responsible for the phosphorylation of NRI. It has been shown that NRII also has the ability to dephosphorylate NRI-phosphate but only when PII is present at a concentration greatly in excess of that of NRII. We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI-phosphate when present in equimolar concentration with NRII. This effect of glutamate appears to be a backup control that becomes effective when the normal regulation of PII activity is disabled.