Open AccessCloning and characterization of the glucokinase gene of Brucella abortus 19 and identification of three other genes
Author(s) -
Richard C. Essenberg
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.21.6297-6300.1995
Subject(s) - biology , open reading frame , gene , cloning (programming) , escherichia coli , strain (injury) , clone (java method) , brucella , molecular cloning , genetics , glucokinase , identification (biology) , peptide sequence , nucleic acid sequence , microbiology and biotechnology , brucellosis , virology , anatomy , computer science , programming language , botany
A clone from Brucella abortus 19 complemented an Escherichia coli strain deficient in phosphorylation of glucose. Open reading frames similar to E. coli mepA, glk, and genes encoding ATP-coupled exporters were found in the sequence. A fourth affected growth on minimal media of the ptsI glk strain with various carbon sources.