Open AccessFunctional analysis of sequences adjacent to dapE of Corynebacterium glutamicum reveals the presence of aroP, which encodes the aromatic amino acid transporter
Author(s) -
Axel Wehrmann,
Schirin Morakkabati,
Reinhard Krämer,
Hermann Sahm,
Lothar Eggeling
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.20.5991-5993.1995
Subject(s) - corynebacterium glutamicum , biology , lysine , aromatic amino acids , mutant , biochemistry , amino acid , gene , molecular cloning , corynebacterium , biosynthesis , dna , locus (genetics) , genetics , peptide sequence , bacteria
An initially nonclonable DNA locus close to a gene of L-lysine biosynthesis in Corynebacterium glutamicum was analyzed in detail. Its stepwise cloning and its functional identification by monitoring the amino acid uptakes of defined mutants, together with mechanistic studies, identified the corresponding structure as aroP, the general aromatic amino acid uptake system.