Open AccessIdentification of channel-forming activity in the cell wall of Corynebacterium glutamicum
Author(s) -
Michael Niederweis,
Elke Maier,
Thomas Lichtinger,
Roland Benz,
Reinhard Krämer
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.19.5716-5718.1995
Subject(s) - corynebacterium glutamicum , biology , corynebacterium , ion channel , cell wall , conductance , biophysics , biochemistry , diffusion , ion , bacteria , chemistry , genetics , gene , organic chemistry , receptor , mathematics , physics , combinatorics , thermodynamics
The cell wall of the gram-positive Corynebacterium glutamicum was prepared. It contained an ion-permeable channel with a single-channel conductance of about 6 nS in 1 M KCl. The mobility sequence of the ions in the channel is similar to that in the aqueous phase, suggesting that it is a water-filled channel wide enough to allow unhindered diffusion of ions. The results indicate that we have identified the hydrophilic pathway through the mycolic acid layer of C. glutamicum.