Open AccessCharacterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase
Author(s) -
Elarbi Haddaoui,
MarieFrançoise PetitGlatron,
Régis Chambert
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.17.5148-5150.1995
Subject(s) - polyclonal antibodies , alpha amylase , biology , bacillus subtilis , amylase , alpha (finance) , microbiology and biotechnology , biochemistry , in vitro , bacillales , bacillaceae , enzyme , antibody , bacteria , immunology , medicine , construct validity , genetics , nursing , patient satisfaction
Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.