Open AccessIdentification of Pseudomonas aeruginosa glpM, whose gene product is required for efficient alginate biosynthesis from various carbon sources
Author(s) -
Herbert P. Schweizer,
Cecilia Po,
Mateja Bačić
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.16.4801-4804.1995
Subject(s) - biology , biosynthesis , gene , biochemistry , pseudomonas aeruginosa , gene product , sigma factor , protein biosynthesis , dehydrogenase , pseudomonas , amino acid , microbiology and biotechnology , rna , gene expression , rna polymerase , enzyme , bacteria , genetics
In a mucB (algN) genetic background, insertion of an omega element approximately 200 bp downstream of glpD, encoding sn-glycerol-3-phosphate dehydrogenase from Pseudomonas aeruginosa, had an adverse effect on alginate biosynthesis from various carbon sources. The insertion inactivated glpM, a gene encoding a 12,040-M(r) hydrophobic protein containing 109 amino acids. This protein, which was expressed in a T7 RNA polymerase expression system, appears to be a cytoplasmic membrane protein.