Open AccessFunctional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius
Author(s) -
Krishnamurthy Madduri,
C. Richard Hutchinson
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.13.3879-3884.1995
Subject(s) - biology , gene cluster , daunorubicin , gene , streptomyces , open reading frame , biosynthesis , biochemistry , microbiology and biotechnology , genetics , peptide sequence , bacteria , leukemia
Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.