Open AccessItaconate biosynthesis in Aspergillus terreus
Author(s) -
Pascal Bonnarme,
Brigitte Gillet,
AnneMarie Sepulchre,
Catherine Role,
Jean Claude Beloeil,
Claire Ducrocq
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.12.3573-3578.1995
Subject(s) - aspergillus terreus , biosynthesis , biology , biochemistry , metabolic pathway , itaconic acid , nuclear magnetic resonance spectroscopy , yield (engineering) , metabolism , citric acid cycle , enzyme , stereochemistry , chemistry , organic chemistry , materials science , metallurgy , copolymer , polymer
Itaconate biosynthesis was studied in intact cells of high-yield (RC4') and low-yield (CM85J) strains of the fungus Aspergillus terreus by methods (tracers, nuclear magnetic resonance spectroscopy, and mass spectroscopy) that did not interfere with metabolism. Itaconate formation in RC4' required de novo protein biosynthesis. Krebs cycle intermediates increased in both strains during the production of itaconic acid. The Embden-Meyerhof-Parnas pathway and the Krebs cycle were shown to be involved in this biosynthesis by using 14C- and 13C-labelled substrates and nuclear magnetic resonance spectroscopy. A metabolic pathway for itaconate formation from glucose in A. terreus is proposed.