Open AccessReconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase
Author(s) -
Jacky L. Snoep,
N Arfman,
Lorraine P. Yomano,
Ronda Fliege,
Tyrrell Conway,
L. O. Ingram
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.7.2133-2135.1994
Subject(s) - zymomonas mobilis , glucokinase , biology , biochemistry , escherichia coli , snf3 , glucose uptake , mutant , glucose transporter , carbohydrate metabolism , gene , fermentation , insulin , ethanol fuel , microbiology and biotechnology , saccharomyces cerevisiae
Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medium and for acid production on glucose-MacConkey agar plates.