Open AccessMapping and cloning of gldA, the structural gene of the Escherichia coli glycerol dehydrogenase
Author(s) -
Verónica Truniger,
Winfried Boos
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.6.1796-1800.1994
Subject(s) - escherichia coli , biology , operon , glycerol , alcohol dehydrogenase , structural gene , cloning (programming) , gene , biochemistry , molecular cloning , microbiology and biotechnology , nad+ kinase , dehydrogenase , genetics , enzyme , gene expression , computer science , programming language
gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map of E. coli was determined. The expression of gldA was induced by hydroxyacetone under stationary-phase growth conditions.