Open AccessOne-ended insertion of IS911
Author(s) -
Patrice Polard,
Laurent Seroude,
Olivier Fayet,
Marie Françoise Prère,
Michaël Chandler
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.4.1192-1196.1994
Subject(s) - biology , transposase , insertion sequence , mutagenesis , inverted repeat , genetics , transposable element , p element , sequence (biology) , dna , gene , computational biology , mutation , genome
An apparently nonreplicative integration reaction mediated by the insertion sequence IS911 has been analyzed. It is shown to involve the right-end inverted repeat (IRR) of the element and sequences in the flanking vector DNA. The flanking sequences appear to behave as a surrogate IS911 end, since integration is greatly reduced when limited similarities with IRR are eliminated by site-directed mutagenesis. Data are presented which suggest that the activity of the IRR junction results from the proximity of the transposase gene and may therefore reflect preferential transposase recognition of IRR in cis.