Open AccessCloning and analysis of a locus (mcr) involved in mitomycin C resistance in Streptomyces lavendulae
Author(s) -
Paul R. August,
Michael C. Flickinger,
David H. Sherman
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.14.4448-4454.1994
Subject(s) - biology , operon , gene , genetics , mitomycin c , streptomyces , locus (genetics) , streptomycetaceae , cloning (programming) , peptide sequence , sequence analysis , molecular cloning , microbiology and biotechnology , escherichia coli , bacteria , actinomycetales , computer science , programming language
Two genes (mcrA and mcrB) from Streptomyces lavendulae that together confer resistance to mitomycin C were identified. This DNA appears to comprise a polycistronic operon with a drug-inducible leaderless mRNA. The deduced amino acid sequence of mcrA shows similarity to sequences of a special class of bacterial, plant, and animal oxygen oxidoreductases.